Engineered microbial biosensors based on bacterial two-component systems as synthetic biotechnology platforms in bioremediation and biorefinery
نویسندگان
چکیده
Two-component regulatory systems (TCRSs) mediate cellular response by coupling sensing and regulatory mechanisms. TCRSs are comprised of a histidine kinase (HK), which serves as a sensor, and a response regulator, which regulates expression of the effector gene after being phosphorylated by HK. Using these attributes, bacterial TCRSs can be engineered to design microbial systems for different applications. This review focuses on the current advances in TCRS-based biosensors and on the design of microbial systems for bioremediation and their potential application in biorefinery.
منابع مشابه
Biological Applications of Bacterial Nano-Surface Layers : A Brief Overview
Surface layer as the outer protective coverage of bacteria and archaea are two-dimensional crystalline and symmetrical arrays of proteins that recently attract a lot of attention for biologist scientists. The surface layers of bacteria are usually 5 to 10 nm in diameter and represent highly porous protein lattices with uniform size and morphology with the pore sizes of 2 to 8 nm. The crucial an...
متن کاملAdvancing bacteriophage-based microbial diagnostics with synthetic biology.
Figure 1. Engineered bacteriophages can be assembled into sensitive, specific, and rapid microbial diagnostics. (A) Bacteriophages can be designed to express reporter payloads during specific microbial infection, leading to the production of detectable outputs. (B) Designing synthetic bacteriophages as microbial Synthetic biology is an emerging engineering field focused on designing artificial ...
متن کاملField applications of genetically engineered microorganisms for bioremediation processes.
Genetically engineered microorganisms (GEMs) have shown potential for bioremediation applications in soil, groundwater, and activated sludge environments, exhibiting enhanced degradative capabilities encompassing a wide range of chemical contaminants. However, the vast majority of studies pertaining to genetically engineered microbial bioremediation are supported by laboratory-based experimenta...
متن کاملIntroduction, synthesis procedures, and applications of organic-inorganic hybrid nanoflowers in biosciences
Organic-inorganic hybrid nanoflowers with flower-like morphology are new nanostructures comprising organic and inorganic components. In general, the organic component of hybrid nanoflowers mostly consists of proteins, DNA, RNA, plant extracts, metabolites, and natural polymers; and the inorganic component composes of various metal phosphates, including copper, calcium, manganese, iron, zinc, co...
متن کاملBacterial responses to environmental herbicide pollutants (glyphosate and paraquat)
The toxic effect of herbicides on non- target microorganisms may influence degradation of organic matter resulting in changes to nutrient cycling. In the present study, different strains of bacteria incubated in media containing different concentrations of glyphosate and paraquat were assessed over a period of two incubation terms. The deleterious impact of the herbicide was observed as glyphos...
متن کامل